Brief intervention of smoking cessation by health professionals has been recommended worldwide. However, this practice has not been fully achieved in dental setting. The present study aimed to examine training course to pilot implementation of the WHO policy recommendations on integrating brief tobacco interventions into oral health program regarding smoking cessation for current smokers and to avoid exposure to secondhand smoke for non-smokers. We held a 1-day training course for dental professionals in Japan. The training course was organized based on the training package for primary care providers, which aims to assist countries in taking one of their first steps towards providing comprehensive tobacco dependence treatment to all tobacco users by integrating brief tobacco interventions (brief advice) into primary care. As a total, 55 oral professionals, 32 dentists and 23 dental hygienists from 19 private clinics and one university hospital, completed the training course.